Interchangeability of a Refractometric and Biuret Method for Serum Total Proteins
نویسندگان
چکیده
منابع مشابه
The measurement of total serum proteins by the Biuret method.
The biuret reaction for proteins provides a simple and precise method for measuring serum proteins; Beer's law is obeyed to at least 10 g per dl. Several stable biuret reagents are available. Hemoglobin is the only important cause of interference which cannot be minimized by use of a sample blank. The mechanism of the biuret reaction is described and attention is drawn to the heterogeneity of t...
متن کاملNew refractometric methods for the determination of total proteins in serum and in urine.
New, precise, physical methods for the determination of total protein in serum or plasma and in urine are described. They are based upon the refractometric determination of total solids in these fluids before and after protein has been removed by coagulation and, in ordinary use, they require no special standardization or calibration. The urine method is applicable primarily to protein concentr...
متن کاملDextran interference in biuret-based assays for serum proteins.
We found the Easy very easy to run and without te#{233}hnique-dependent steps in the procedure. Operators need not have a high level of skill. Calibration is stable for one month, except for the ion-selective electrodes, which must be calibrated every two days. In conclusion, we found the Easy satisfactory in operational and analytical performance. The analyzer is well suited for use in the eme...
متن کاملDetermination of serum proteins by means of the biuret reaction.
In the course of an investigation of the biochemical changes following experimental liver injury we felt the need of a simple, rapid, and accurate method for determining the protein fractions in small amounts of serum. Among the simpler procedures known, the biuret reaction seemed to offer the most encouraging possibilities. Variations and improvements in the application of the biuret reaction ...
متن کاملAssessment of a biuret method without concentration step for total protein determination in cerebrospinal fluid.
E xamination of cerebrospinal fluid (CSF) total protein is used chiefly to detect increased permeabili ty of the blood-brain barrier to plasma proteins or increased intrathecal secretion of immunoglobulins (1). A rapid and quite precise method is required. The biuret method is considered as a potential reference method for total protein determination in biological fluids (2-4). It has been modi...
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ژورنال
عنوان ژورنال: Proceedings of the Association of Clinical Biochemists
سال: 1965
ISSN: 0369-8564
DOI: 10.1177/036985646500300801